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Sustainable & Synthetic Biotechnology Group
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Publications

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Engineered production of isoprene from the model green microalga Chlamydomonas reinhardtii
Article Year: 2023 DOI:10.1016/j.mec.2023.e00221
Authors: Razan Z. Yahya, Gordon B. Wellman, Sebastian Overmans, Kyle J. Lauersen
  • Isoprene
  • Microalgae
  • Chlamydomonas reinhardtii
  • MEP pathway
  • volatile carbon
Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii
Article Year: 2023 DOI:10.1021/acssynbio.2c00616
Authors: Sofia Amendola, Jacob S. Kneip, Florian Meyer, Federico Perozeni, Stefano Cazzaniga, Kyle J. Lauersen, Matteo Ballottari, Thomas Baier
  • Biosynthesis
  • Dyes and pigments
  • Genetics
  • Hydrocarbons
  • Peptides and proteins
PlantACT! – how to tackle the climate crisis
Opinion Paper Year: 2023 DOI:10.1016/j.tplants.2023.01.005
Authors: Heribert Hirt, Salim Al-Babili, Marilia Almeida-Trapp, Martin Antoine, Manuel Aranda, Dorothea Bartels, Malcolm Bennett, Ikram Blilou, Damian Boer, Alix Boulouis, Chris Bowler, Sophie Brunel-Muguet, Fabien Chardon, Jean Colcombet, Vincent Colot, Agata Daszkowska-Golec, Jose R. Dinneny, Ben Field, Katja Froehlich, Catherine H. Gardener, Alain Gojon, Eric Gomès, Eva María Gómez Álvarez, Crisanto Gutierrez, Michel Havaux, Scott Hayes, Edith Heard, Michael Hodges, Amal Khalaf Alghamdi, Laurent Laplaze, Kyle J. Lauersen, Nathalie Leonhard, Xenie Johnson, Jonathan Jones, Hannes Kollist, Stanislav Kopriva, Anne Krapp, Mauricio Lopez-Portillo Masson, Matthew F. McCabe, Livia Merendino, Antonio Molina, Jose L. Moreno Ramirez, Bernd Müller-Röber, Michaël Nicolas, Ido Nir, Izamar Olivas Orduna, José Pardo-Tomás, Jean-Philippe Reichheld, Pedro Luis Rodriguez Egea, Hatem Rouached, Maged M. Saad, Peter Schlögelhofer, Kirti A. Singh, Ive De Smet, Clara Stanschewski, Alice Stra, Mark Tester, Catherine Walshe, Andreas P.M. Weber, Detlef Weigel, Philip Wigge, Michael Wrzaczek, Brande Wulff, Iain M. Young
  • greenhouse gas emission
  • climate change
  • agriculture
  • food safety
  • water use efficiency
  • nitrogen use efficiency
  • carbon sequestration
Biomass generation and heterologous isoprenoid milking from engineered microalgae grown in anaerobic membrane bioreactor effluent
Article Year: 2023 DOI:10.1016/j.watres.2022.119486
Authors: Barbara Bastos de Freitas, Sebastian Overmans, Julie Sanchez Medina, Pei-Ying Hong, Kyle J. Lauersen
  • Chlamydomonas reinhardtii
  • patchoulol
  • Terpenoids
  • Wastewater treatment
  • CO2 capture
  • Anaerobic membrane bioreactor (AnMBR)
Teaching an old ‘doc’ new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection
Article Year: 2022 DOI:10.3389/fbioe.2022.979607
Authors: Sergio Gutiérrez, Gordon B. Wellman, Kyle J. Lauersen
  • fluorescent protein
  • Microalgae
  • fluorescence
  • molecular tools
  • epitope tags
Farnesyl pyrophosphate compartmentalization in the green microalga Chlamydomonas reinhardtii during heterologous (E)-α-bisabolene production
Article Year: 2022 DOI:10.1186/s12934-022-01910-5
Authors: Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse
  • Microalgae
  • Chlamydomonas reinhardtii
  • Terpenoids
  • Isoprenoids
  • Bisabolene
Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways
Review Year: 2022 DOI:10.1016/j.jare.2022.07.011
Authors: Sulamita Santos Correa, Junia Schultz, Kyle J. Lauersen, Alexandre Soares Rosado
  • Carbon fixation
  • Autotrophic
  • Thermophiles
  • Omics
  • Biochemistry
  • Synthetic pathway
A New Approach to the Study of Plastidial Stress Granules: The Integrated Use of Arabidopsis thaliana and Chlamydomonas reinhardtii as Model Organisms
Opinion Paper Year: 2022 DOI:10.3390/plants11111467
Authors: Fareena Rafique, Kyle J. Lauersen, Monika Chodasiewicz, Nicolás E. Figueroa
  • plant stress granules
  • chloroplast
  • plant stress response
  • Chlamydomonas reinhardtii
  • Arabidopsis
  • abiotic stress
  • liquid–liquid phase separation
Chlamydomonas reinhardtii Alternates Peroxisomal Contents in Response to Trophic Conditions
Article Year: 2022 DOI:10.3390/cells11172724
Authors: Naohiro Kato, Clayton McCuiston, Kimberly A. Szuska, Kyle J. Lauersen, Gabela Nelson, Alexis Strain
  • Chlamydomonas reinhardtii
  • Microalgae
  • fluorescence microscopy
  • peroxisomes
  • Fatty acids
  • beta-oxidation
  • glyoxylate cycle enzymes
  • PEX
  • PTS1
Combinatorial Engineering Enables Photoautotrophic Growth in High Cell Density Phosphite-Buffered Media to Support Engineered Chlamydomonas reinhardtii Bio-Production Concepts
Article Year: 2022 DOI:10.3389/fmicb.2022.885840
Authors: Malak N. Abdallah, Gordon B. Wellman, Sebastian Overmans, Kyle J. Lauersen
  • Microalgae
  • Phosphite
  • algal biotechnology
  • waste reuse
  • metabolic engineering
  • Isoprenoids
  • Terpenoids
Continuous extraction and concentration of secreted metabolites from engineered microbes using membrane technology
Article Year: 2022 DOI:10.1039/D2GC00938B
Authors: Sebastian Overmans, Gergo Ignacz, Aron K. Beke, Jiajie Xu, Pascal E. Saikaly, Gyorgy Szekely, Kyle J. Lauersen
  • Chlamydomonas reinhardtii
  • hollow-fiber membrane
  • metabolites
  • organic solvent nanofiltration
Biocompatible fluorocarbon liquid underlays for in situ extraction of isoprenoids from microbial cultures
Article Year: 2022 DOI:10.1039/d2ra01112c
Authors: Sebastian Overmans, Kyle J. Lauersen
  • Perfluoro liquids
  • fluorocarbons
  • algal biotechnology
  • Chlamydomonas reinhardtii
  • Terpenoids
Subcellular Localizations of Catalase and Exogenously Added Fatty Acid in Chlamydomonas reinhardtii
Article Year: 2021 DOI:10.3390/cells10081940
Authors: Naohiro Kato, Gabela Nelson, Kyle J. Lauersen
  • Chlamydomonas reinhardtii
  • Microalgae
  • catalase
  • Fatty acids
  • peroxisomes
  • fluorescence microscopy
Intron-mediated enhancement of transgene expression in the oleaginous diatom Fistulifera solaris towards bisabolene production
Short Communication Year: 2021 DOI:10.1016/j.algal.2021.102345
Authors: Tsuyoshi Tanaka, Yoshiaki Maeda, Noraiza Suhaimi, Chiharu Tsuneoka, Tomomi Nonoyama, Tomoko Yoshino, Naohiro Kato, Kyle J. Lauersen
  • Intron
  • Intron mediated enhancement
  • diatom
  • sesquiterpene
  • Bisabolene
Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells
Article Year: 2021 DOI:10.1126/sciadv.abe8349
Authors: Despoina Kerselidou, Bushra Dohai, David R. Nelson, Sarah Daakour, Nicolas De Cock, Zahra Al Oula Hassoun, Dae-Kyum Kim, Julien Olivet, Diana C. El Assal, Ashish Jaiswal, Amnah Alzahmi, Deeya Saha, Charlotte Pain, Filip Matthijssens, Pierre Lemaitre, Michael Herfs, Julien Chapuis, Bart Ghesquiere, Didier Vertommen, Verena Kriechbaumer, Kèvin Knoops, Carmen Lopez-Iglesias, Marc van Zandvoort, Jean-Charles Lambert, Julien Hanson, Christophe Desmet, Marc Thiry, Kyle J. Lauersen, Marc Vidal, Pieter Van Vlierberghe, Franck Dequiedt, Kourosh Salehi-Ashtiani, Jean-Claude Twizere
  • glycosylation
  • endoplasmic reticulum
  • mammalian cells
Engineering Biocatalytic Solar Fuel Production: The PHOTOFUEL Consortium
Article Year: 2021 DOI:10.1016/j.tibtech.2021.01.003
Authors: Julian Wichmann, Kyle J. Lauersen, Natascia Biondi, Magnus Christensen, Tiago Guerra, Klaus Hellgardt, Simon Kühner, Markku Kuronen, Pia Lindberg, Christine Rösch, Ian S. Yunus, Patrik Jones, Peter Lindblad, Olaf Kruse
  • Solar fuel
  • engine tests
  • lifecycle assessment
  • photo-biomanufacturing
  • techno-economic analysis
Gene Delivery Technologies with Applications in Microalgal Genetic Engineering
Review Year: 2021 DOI:10.3390/biology10040265
Authors: Sergio Gutiérrez, Kyle J. Lauersen
  • Microalgae
  • transformation
  • Cyanobacteria
  • DNA
Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites
Review Year: 2021 DOI:10.1021/acssynbio.0c00507
Authors: Muhammad Saad Ahmed, Kyle J. Lauersen, Sana Ikram, Chun Li
  • metabolites
  • cytotoxicity
  • transporter engineering
  • efflux transporters
Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution
Article Year: 2021 DOI:10.1016/j.chom.2020.12.005
Authors: David R. Nelson, Khaled M. Hazzouri, Kyle J. Lauersen, Ashish Jaiswal, Amphun Chaiboonchoe, Alexandra Mystikou, Weiqi Fu, Sarah Daakour, Bushra Dohai, Amnah Alzahmi, David Nobles, Mark Hurd, Julie Sexton, Michael J. Preston, Joan Blanchette, Michael W. Lomas, Khaled M.A. Amiri, Kourosh Salehi-Ashtiani
  • Microalgae
  • Genomics
  • Viruses
  • Halotolerance
  • Chlorophyta
  • Rhodophyta
  • Cercozoa
  • Cryptophyta
  • Haptophyta
  • Ochrophyta
Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii
Journal paper Open Access Year: 2020
Authors: Federico Perozeni, Stefano Cazzaniga, Thomas Baier, Francesca Zanoni, Gianni Zoccatelli, Kyle J. Lauersen, Lutz Wobbe, Matteo Ballottari
  • Chlamydomonas reinhardtii
  • β-carotene ketolase
  • Haematococcus lacustris
  • astaxanthin production
  • tailor-made pigments
Green algal hydrocarbon metabolism is an exceptional source of sustainable chemicals
Open access article Year: 2020
Authors: Julian Wichmann, Kyle J. Lauersen, Olaf Kruse
  • Food Biotechnology
  • Plant Biotechnology
  • Green Cell Factories
  • Hydrocarbons
Intronserter, an advanced online tool for design of intron containing transgenes
Journal paper Year: 2019
Authors: Daniel Jaeger, Thomas Baier, Kyle J. Lauersen
  • Intron mediated enhancement
  • Nuclear transgene expression
  • Chlamydomonas reinhardtii
  • Eukaryotic gene expression
  • Microalgal engineering
Characterization of the GPR1/FUN34/YaaH protein family in the green microalga Chlamydomonas suggests their role as intracellular membrane acetate channels
Open access article Year: 2019
Authors: Lorenzo Durante, Wolfgang Hübner, Kyle J. Lauersen, Claire Remacle
  • acetate transporter
  • Chlamydomonas reinhardtii
  • GFY protein
  • membrane channel
  • microbodies
Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production
Journal paper Year: 2019
Authors: Kyle J. Lauersen
  • Microalgae
  • Chlamydomonas reinhardtii
  • Phaeodactylum tricornutum
  • Terpenoids
  • Isoprenoids
  • Cytochrome
  • P450s
Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii
Article Year: 2018
Authors: Thomas Baier, Dana Kros, Rebecca C. Feiner, Kyle J. Lauersen, Kristian M. Müller, Olaf Kruse
  • Microalgae
  • Chlamydomonas reinhardtii
  • transgene expression
  • recombinant protein secretion
  • hydrophobin
  • detergent-based aqueous two-phase extraction
Synthetic metabolic pathways for photobiological conversion of CO2 into hydrocarbon fuel
Journal paper Year: 2018
Authors: Ian Sofian Yunusa, Julian Wichmann, Robin Wördenweber, Kyle J. Lauersen, Olaf Kruse, Patrik R. Jones
  • Hydrocarbon fuel
  • Algae
  • Cyanobacteria
  • Alkanes
  • Fatty acids
Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii
Article Year: 2018
Authors: Kyle J. Lauersen, Julian Wichmann, Thomas Baier, Sotirios C. Kampranis, Irini Pateraki, Birger Lindberg Møller, Olaf Kruse
  • Microalgae
  • Chlamydomonas reinhardtii
  • Terpenoids
  • Isoprenoids
  • Diterpenoids
  • Cytochrome
  • P450s
Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii
Letter Year: 2018
Authors: Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, Stéphane D. Lemaire
  • algal biotechnology
  • Chlamydomonas reinhardtii
  • modular cloning
  • synthetic biology
Photocatalytic Production of Bisabolene from Green Microalgae Mutant: Process Analysis and Kinetic Modeling
Article Year: 2018
Authors: Irina Harun, Ehecatl Antonio Del Rio-Chanona, Jonathan L. Wagner, Kyle J. Lauersen, Dongda Zhan, Klaus Hellgardt
  • Hydrocarbons
  • Carbon
  • Biomass
  • Light Biofuels
Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum
Article Year: 2018
Authors: Nadja A. Henke, Julian Wichmann, Thomas Baier, Jonas Frohwitter, Kyle J. Lauersen, Joe M. Risse, Petra Peters-Wendisch, Olaf Kruse, Volker F. Wendisch
  • patchoulol
  • Corynebacterium glutamicum
  • sesquiterpene
  • metabolic engineering
  • Algae
  • Escherichia coli
Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii
Journal paper Year: 2018
Authors: Thomas Baier, Julian Wichmann, Olaf Kruse, Kyle J. Lauersen
  • Chlamydomonas reinhardtii
  • Microalgae
Tailored carbon partitioning for phototrophic production of (E)-α-bisabolene from the green microalga Chlamydomonas reinhardtii
Article Year: 2018
Authors: Julian Wichmann, Thomas Baier, Eduard Wentnagel, Kyle J. Lauersen, Olaf Kruse
  • Microalgae
  • Chlamydomonas reinhardtii
  • Terpenoids
  • Bisabolene
  • amiRNA
Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii
Article Year: 2016
Authors: Kyle J. Lauersen, Thomas Baier, Julian Wichmann, Robin Wördenweber, Jan H. Mussgnug, Wolfgang Hübner, Thomas Huser, Olaf Kruse
  • Microalgae
  • Chlamydomonas reinhardtii
  • Terpenoids
  • Sesquiterpenoids
  • patchoulol
Peroxisomal microbodies are at the crossroads of acetate assimilation in the green microalga Chlamydomonas reinhardtii
Article Year: 2016
Authors: Kyle J. Lauersen, Rémi Willamme, Nadine Coosemans, Marine Joris, Olaf Kruse, Claire Remacle
  • Glyoxylate cycle
  • Localization
  • microbodies
  • Isocitrate lyase
  • Acetate assimilation
  • Chlamydomonas reinhardtii
Investigating the dynamics of recombinant protein secretion from a microalgal host
Article Year: 2015
Authors: Kyle J. Lauersen, Isabel Hubera, Julian Wichmann, Thomas Baiera, Andreas Leite, Volker Gaukel, Viktor Kartushin, Anke Rattenholl, Christian Steinweg, Lenavon Riesen, Clemens Posten, Dirk Lütkemeyer, Jan H. Mussgnug, Olaf Kruse
  • Microalgae
  • Lolium perenne ice-binding protein
  • recombinant protein secretion
  • Flat-panel photobioreactor
  • Wave-bag culture
  • Chlamydomonas reinhardtii
Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit
Article Year: 2015
Authors: Kyle J. Lauersen, Olaf Kruse, Jan H. Mussgnug
  • Microalga
  • Chlamydomonas reinhardtii
  • Recombinant proteins
  • Fluorescent proteins
  • Luciferase
  • Protein secretion
Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production
Open access article Year: 2013
Authors: Christian Bogen, Arwa Al-Dilaimi, Andreas Albersmeier, Julian Wichmann, Michael Grundmann, Oliver Rupp, Kyle J. Lauersen, Olga Blifernez-Klassen, Jörn Kalinowski, Alexander Goesmann, Jan H. Mussgnug, Olaf Kruse
  • M. neglectum genome
  • Biofuels
  • Lipid metabolism
  • Neutral lipid accumulation
Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii
Article Year: 2013
Authors: Kyle J. Lauersen, Tara L. Vanderveer, Hanna Berger, Isabell Kaluza, Jan H. Mussgnug, Virginia K. Walker, Olaf Kruse
  • Microalga
  • Lolium perenne ice-binding protein
  • High-value recombinant protein production
  • Chlamydomonas reinhardtii
  • Secretion
Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii
Article Year: 2013
Authors: Kyle J. Lauersen, Hanna Berger, Jan H. Mussgnug, Olaf Kruse
  • Microalgae
  • Enhanced nuclear gene expression
  • Chlamydomonas reinhardtii
  • High-value recombinant protein production
Expression and characterization of an antifreeze protein from the perennial rye grass, Lolium perenne
Article Year: 2011
Authors: Kyle J. Lauersen, Alan Brown, Adam Middleton, Peter L. Davies, Virginia K.Walker
  • Antifreeze protein
  • Lolium perenne
  • Perennial ryegrass
  • Thermal hysteresis
  • Ice recrystallization inhibition
  • Ice-binding proteins
  • Freezing stress response
  • Plant antifreeze protein
  • Freezing tolerance
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