Publications

A rapid CAT transformation protocol and nuclear transgene expression tools for metabolic engineering in Cyanidioschyzon merolae 10D

Article Year: 2024 DOI:10.1016/j.nbt.2024.12.001

Authors: Melany Villegas-Valencia, Martha R. Stark, Mark Seger, Gordon B. Wellman, Sebastian Overmans, Peter J. Lammers, Stephen D. Rader, Kyle J. Lauersen

Cyanidioschyzon merolae 10D
Polyextremophile
Genetic engineering
Fluorescent reporters
Transformation
Isoprene

Compartmentalized Sesquiterpenoid Biosynthesis and Functionalization in the Chlamydomonas reinhardtii Plastid

Article Year: 2024 DOI:10.1002/cbic.202400902

Authors: Sergio Gutiérrez, Sebastian Overmans, Gordon B. Wellman, Kyle J. Lauersen

Chlamydomonas reinhardtii
Chloroplast
Cytochrome P450
Metabolic engineering
Terpenoid biosynthesis

Green extraction of pigment from astaxanthin-producing algae using natural deep eutectic solvents

Article Year: 2024 DOI:10.1016/j.algal.2024.103668

Authors: Lamya Al Fuhaid, Gordon B. Wellman, Najeh Kharbatia, Andreia S.F. Farinha, Johannes S. Vrouwenvelder, Rob Verpoorte, Young Hae Choi, Geert-Jan Witkamp, Kyle J. Lauersen, Luca Fortunato

Astaxanthin
Algae
NADES
Food-grade

Bicistronic expression of nuclear transgenes in Chlamydomonas reinhardtii

Article Year: 2024 DOI:10.1111/tpj.16677

Authors: Nick Jacobebbinghaus, Kyle J. Lauersen, Olaf Kruse, Thomas Baier

bicistronic gene expression
polycistron
nuclear transgene engineering
transgene design
Microalgae
Chlamydomonas reinhardtii

The synthetic future of algal genomes

Review Year: 2024 DOI:10.1016/j.xgen.2024.100505

Authors: Hugh D. Goold, Jeffrey L. Moseley, Kyle J. Lauersen

synthetic biology
synthetic genomics
algal biotechnology
metabolic engineering
engineering biology
light-driven biotechnology

A synthetic biology and green bioprocess approach to recreate agarwood sesquiterpenoid mixtures

Article Year: 2024 DOI:10.1039/D3GC03708H

Authors: Sergio Gutiérrez, Sebastian Overmans, Gordon B. Wellman, Vasilios G. Samaras, Claudia Oviedo, Martin Gede, Gyorgy Szekely, Kyle J. Lauersen

Agarwood
Aquilaria sp.
Green algae
Chlamydomonas reinhardtii
Terpenoids
metabolic engineering
Green Chemistry
organic solvent nanofiltration

Engineered ketocarotenoid biosynthesis in the polyextremophilic red microalga Cyanidioschyzon merolae 10D

Article Year: 2023 DOI:10.1016/j.mec.2023.e00226

Authors: Mark Seger, Fakhriyya Mammadova, Melany Villegas-Valencia, Barbara Bastos de Freitas, Clarissa Chang, Iona Isachsen, Haley Hemstreet, Fatimah Abualsaud, Malia Boring, Peter J. Lammers, Kyle J. Lauersen

Microalgae
Red algae
ketocarotenoids
Cyanidiophyceae
polyextremophiles
astaxanthin

Crude algal biomass for the generation of thin-film composite solvent-resistant nanofiltration membranes

Article Year: 2023 DOI:10.1016/j.cej.2023.144153

Authors: Cong Yang, Joyce Cavalcante, Barbara Bastos de Freitas, Kyle J. Lauersen, Gyorgy Szekely

Algae
Thin-film composite
Biomass
Upcycling
Green solvent
Sustainability

Cultivation of the polyextremophile Cyanidioschyzon merolae 10D during summer conditions on the coast of the Red Sea and its adaptation to hypersaline sea water

Article Year: 2023 DOI:10.3389/fmicb.2023.1157151

Authors: Melany Villegas-Valencia, Ricardo E. González-Portela, Bárbara Bastos de Freitas, Abdulaziz Al Jahdali, Gabriel I. Romero-Villegas, Raghdah Malibari, Rahul Vijay Kapoore, Claudio Fuentes-Grünewald, Kyle J. Lauersen

circular resource biotechnology
desert
Cyanidioschyzon merolae 10D
sea water
Saudi Arabia Red Sea coast
algal biotechnology

Engineered production of isoprene from the model green microalga Chlamydomonas reinhardtii

Article Year: 2023 DOI:10.1016/j.mec.2023.e00221

Authors: Razan Z. Yahya, Gordon B. Wellman, Sebastian Overmans, Kyle J. Lauersen

Isoprene
Microalgae
Chlamydomonas reinhardtii
MEP pathway
volatile carbon

Metabolic Engineering for Efficient Ketocarotenoid Accumulation in the Green Microalga Chlamydomonas reinhardtii

Article Year: 2023 DOI:10.1021/acssynbio.2c00616

Authors: Sofia Amendola, Jacob S. Kneip, Florian Meyer, Federico Perozeni, Stefano Cazzaniga, Kyle J. Lauersen, Matteo Ballottari, Thomas Baier

Biosynthesis
Dyes and pigments
Genetics
Hydrocarbons
Peptides and proteins

PlantACT! – how to tackle the climate crisis

Opinion Paper Year: 2023 DOI:10.1016/j.tplants.2023.01.005

Authors: Heribert Hirt, Salim Al-Babili, Marilia Almeida-Trapp, Martin Antoine, Manuel Aranda, Dorothea Bartels, Malcolm Bennett, Ikram Blilou, Damian Boer, Alix Boulouis, Chris Bowler, Sophie Brunel-Muguet, Fabien Chardon, Jean Colcombet, Vincent Colot, Agata Daszkowska-Golec, Jose R. Dinneny, Ben Field, Katja Froehlich, Catherine H. Gardener, Alain Gojon, Eric Gomès, Eva María Gómez Álvarez, Crisanto Gutierrez, Michel Havaux, Scott Hayes, Edith Heard, Michael Hodges, Amal Khalaf Alghamdi, Laurent Laplaze, Kyle J. Lauersen, Nathalie Leonhard, Xenie Johnson, Jonathan Jones, Hannes Kollist, Stanislav Kopriva, Anne Krapp, Mauricio Lopez-Portillo Masson, Matthew F. McCabe, Livia Merendino, Antonio Molina, Jose L. Moreno Ramirez, Bernd Müller-Röber, Michaël Nicolas, Ido Nir, Izamar Olivas Orduna, José Pardo-Tomás, Jean-Philippe Reichheld, Pedro Luis Rodriguez Egea, Hatem Rouached, Maged M. Saad, Peter Schlögelhofer, Kirti A. Singh, Ive De Smet, Clara Stanschewski, Alice Stra, Mark Tester, Catherine Walshe, Andreas P.M. Weber, Detlef Weigel, Philip Wigge, Michael Wrzaczek, Brande Wulff, Iain M. Young

greenhouse gas emission
climate change
agriculture
food safety
water use efficiency
nitrogen use efficiency
carbon sequestration

Biomass generation and heterologous isoprenoid milking from engineered microalgae grown in anaerobic membrane bioreactor effluent

Article Year: 2023 DOI:10.1016/j.watres.2022.119486

Authors: Barbara Bastos de Freitas, Sebastian Overmans, Julie Sanchez Medina, Pei-Ying Hong, Kyle J. Lauersen

Chlamydomonas reinhardtii
patchoulol
Terpenoids
Wastewater treatment
CO2 capture
Anaerobic membrane bioreactor (AnMBR)

Teaching an old ‘doc’ new tricks for algal biotechnology: Strategic filter use enables multi-scale fluorescent protein signal detection

Article Year: 2022 DOI:10.3389/fbioe.2022.979607

Authors: Sergio Gutiérrez, Gordon B. Wellman, Kyle J. Lauersen

fluorescent protein
Microalgae
fluorescence
molecular tools
epitope tags

Farnesyl pyrophosphate compartmentalization in the green microalga Chlamydomonas reinhardtii during heterologous (E)-α-bisabolene production

Article Year: 2022 DOI:10.1186/s12934-022-01910-5

Authors: Julian Wichmann, Annibel Eggert, Liam D. H. Elbourne, Ian T. Paulsen, Kyle J. Lauersen, Olaf Kruse

Microalgae
Chlamydomonas reinhardtii
Terpenoids
Isoprenoids
Bisabolene

Natural carbon fixation and advances in synthetic engineering for redesigning and creating new fixation pathways

Review Year: 2022 DOI:10.1016/j.jare.2022.07.011

Authors: Sulamita Santos Correa, Junia Schultz, Kyle J. Lauersen, Alexandre Soares Rosado

Carbon fixation
Autotrophic
Thermophiles
Omics
Biochemistry
Synthetic pathway

A New Approach to the Study of Plastidial Stress Granules: The Integrated Use of Arabidopsis thaliana and Chlamydomonas reinhardtii as Model Organisms

Opinion Paper Year: 2022 DOI:10.3390/plants11111467

Authors: Fareena Rafique, Kyle J. Lauersen, Monika Chodasiewicz, Nicolás E. Figueroa

plant stress granules
chloroplast
plant stress response
Chlamydomonas reinhardtii
Arabidopsis
abiotic stress
liquid–liquid phase separation

Chlamydomonas reinhardtii Alternates Peroxisomal Contents in Response to Trophic Conditions

Article Year: 2022 DOI:10.3390/cells11172724

Authors: Naohiro Kato, Clayton McCuiston, Kimberly A. Szuska, Kyle J. Lauersen, Gabela Nelson, Alexis Strain

Chlamydomonas reinhardtii
Microalgae
fluorescence microscopy
peroxisomes
Fatty acids
beta-oxidation
glyoxylate cycle enzymes
PEX
PTS1

Combinatorial Engineering Enables Photoautotrophic Growth in High Cell Density Phosphite-Buffered Media to Support Engineered Chlamydomonas reinhardtii Bio-Production Concepts

Article Year: 2022 DOI:10.3389/fmicb.2022.885840

Authors: Malak N. Abdallah, Gordon B. Wellman, Sebastian Overmans, Kyle J. Lauersen

Microalgae
Phosphite
algal biotechnology
waste reuse
metabolic engineering
Isoprenoids
Terpenoids

Continuous extraction and concentration of secreted metabolites from engineered microbes using membrane technology

Article Year: 2022 DOI:10.1039/D2GC00938B

Authors: Sebastian Overmans, Gergo Ignacz, Aron K. Beke, Jiajie Xu, Pascal E. Saikaly, Gyorgy Szekely, Kyle J. Lauersen

Chlamydomonas reinhardtii
hollow-fiber membrane
metabolites
organic solvent nanofiltration

Biocompatible fluorocarbon liquid underlays for in situ extraction of isoprenoids from microbial cultures

Article Year: 2022 DOI:10.1039/d2ra01112c

Authors: Sebastian Overmans, Kyle J. Lauersen

Perfluoro liquids
fluorocarbons
algal biotechnology
Chlamydomonas reinhardtii
Terpenoids

Subcellular Localizations of Catalase and Exogenously Added Fatty Acid in Chlamydomonas reinhardtii

Article Year: 2021 DOI:10.3390/cells10081940

Authors: Naohiro Kato, Gabela Nelson, Kyle J. Lauersen

Chlamydomonas reinhardtii
Microalgae
catalase
Fatty acids
peroxisomes
fluorescence microscopy

Intron-mediated enhancement of transgene expression in the oleaginous diatom Fistulifera solaris towards bisabolene production

Short Communication Year: 2021 DOI:10.1016/j.algal.2021.102345

Authors: Tsuyoshi Tanaka, Yoshiaki Maeda, Noraiza Suhaimi, Chiharu Tsuneoka, Tomomi Nonoyama, Tomoko Yoshino, Naohiro Kato, Kyle J. Lauersen

Intron
Intron mediated enhancement
diatom
sesquiterpene
Bisabolene

Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells

Article Year: 2021 DOI:10.1126/sciadv.abe8349

Authors: Despoina Kerselidou, Bushra Dohai, David R. Nelson, Sarah Daakour, Nicolas De Cock, Zahra Al Oula Hassoun, Dae-Kyum Kim, Julien Olivet, Diana C. El Assal, Ashish Jaiswal, Amnah Alzahmi, Deeya Saha, Charlotte Pain, Filip Matthijssens, Pierre Lemaitre, Michael Herfs, Julien Chapuis, Bart Ghesquiere, Didier Vertommen, Verena Kriechbaumer, Kèvin Knoops, Carmen Lopez-Iglesias, Marc van Zandvoort, Jean-Charles Lambert, Julien Hanson, Christophe Desmet, Marc Thiry, Kyle J. Lauersen, Marc Vidal, Pieter Van Vlierberghe, Franck Dequiedt, Kourosh Salehi-Ashtiani, Jean-Claude Twizere

glycosylation
endoplasmic reticulum
mammalian cells

Engineering Biocatalytic Solar Fuel Production: The PHOTOFUEL Consortium

Article Year: 2021 DOI:10.1016/j.tibtech.2021.01.003

Authors: Julian Wichmann, Kyle J. Lauersen, Natascia Biondi, Magnus Christensen, Tiago Guerra, Klaus Hellgardt, Simon Kühner, Markku Kuronen, Pia Lindberg, Christine Rösch, Ian S. Yunus, Patrik Jones, Peter Lindblad, Olaf Kruse

Solar fuel
engine tests
lifecycle assessment
photo-biomanufacturing
techno-economic analysis

Gene Delivery Technologies with Applications in Microalgal Genetic Engineering

Review Year: 2021 DOI:10.3390/biology10040265

Authors: Sergio Gutiérrez, Kyle J. Lauersen

Microalgae
transformation
Cyanobacteria
DNA

Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites

Review Year: 2021 DOI:10.1021/acssynbio.0c00507

Authors: Muhammad Saad Ahmed, Kyle J. Lauersen, Sana Ikram, Chun Li

metabolites
cytotoxicity
transporter engineering
efflux transporters

Large-scale genome sequencing reveals the driving forces of viruses in microalgal evolution

Article Year: 2021 DOI:10.1016/j.chom.2020.12.005

Authors: David R. Nelson, Khaled M. Hazzouri, Kyle J. Lauersen, Ashish Jaiswal, Amphun Chaiboonchoe, Alexandra Mystikou, Weiqi Fu, Sarah Daakour, Bushra Dohai, Amnah Alzahmi, David Nobles, Mark Hurd, Julie Sexton, Michael J. Preston, Joan Blanchette, Michael W. Lomas, Khaled M.A. Amiri, Kourosh Salehi-Ashtiani

Microalgae
Genomics
Viruses
Halotolerance
Chlorophyta
Rhodophyta
Cercozoa
Cryptophyta
Haptophyta
Ochrophyta

Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii

Journal paper Open Access Year: 2020

Authors: Federico Perozeni, Stefano Cazzaniga, Thomas Baier, Francesca Zanoni, Gianni Zoccatelli, Kyle J. Lauersen, Lutz Wobbe, Matteo Ballottari

Chlamydomonas reinhardtii
β-carotene ketolase
Haematococcus lacustris
astaxanthin production
tailor-made pigments

Green algal hydrocarbon metabolism is an exceptional source of sustainable chemicals

Open access article Year: 2020

Authors: Julian Wichmann, Kyle J. Lauersen, Olaf Kruse

Food Biotechnology
Plant Biotechnology
Green Cell Factories
Hydrocarbons

Intronserter, an advanced online tool for design of intron containing transgenes

Journal paper Year: 2019

Authors: Daniel Jaeger, Thomas Baier, Kyle J. Lauersen

Intron mediated enhancement
Nuclear transgene expression
Chlamydomonas reinhardtii
Eukaryotic gene expression
Microalgal engineering

Characterization of the GPR1/FUN34/YaaH protein family in the green microalga Chlamydomonas suggests their role as intracellular membrane acetate channels

Open access article Year: 2019

Authors: Lorenzo Durante, Wolfgang Hübner, Kyle J. Lauersen, Claire Remacle

acetate transporter
Chlamydomonas reinhardtii
GFY protein
membrane channel
microbodies

Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production

Journal paper Year: 2019

Authors: Kyle J. Lauersen

Microalgae
Chlamydomonas reinhardtii
Phaeodactylum tricornutum
Terpenoids
Isoprenoids
Cytochrome
P450s

Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii

Article Year: 2018

Authors: Thomas Baier, Dana Kros, Rebecca C. Feiner, Kyle J. Lauersen, Kristian M. Müller, Olaf Kruse

Microalgae
Chlamydomonas reinhardtii
transgene expression
recombinant protein secretion
hydrophobin
detergent-based aqueous two-phase extraction

Synthetic metabolic pathways for photobiological conversion of CO2 into hydrocarbon fuel

Journal paper Year: 2018

Authors: Ian Sofian Yunusa, Julian Wichmann, Robin Wördenweber, Kyle J. Lauersen, Olaf Kruse, Patrik R. Jones

Hydrocarbon fuel
Algae
Cyanobacteria
Alkanes
Fatty acids

Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii

Article Year: 2018

Authors: Kyle J. Lauersen, Julian Wichmann, Thomas Baier, Sotirios C. Kampranis, Irini Pateraki, Birger Lindberg Møller, Olaf Kruse

Microalgae
Chlamydomonas reinhardtii
Terpenoids
Isoprenoids
Diterpenoids
Cytochrome
P450s

Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii

Letter Year: 2018

Authors: Pierre Crozet, Francisco J. Navarro, Felix Willmund, Payam Mehrshahi, Kamil Bakowski, Kyle J. Lauersen, Maria-Esther Pérez-Pérez, Pascaline Auroy, Aleix Gorchs Rovira, Susana Sauret-Gueto, Justus Niemeyer, Benjamin Spaniol, Jasmine Theis, Raphael Trösch, Lisa-Desiree Westrich, Konstantinos Vavitsas, Thomas Baier, Wolfgang Hübner, Felix de Carpentier, Mathieu Cassarini, Antoine Danon, Julien Henri, Christophe H. Marchand, Marcello de Mia, Kevin Sarkissian, David C. Baulcombe, Gilles Peltier, José-Luis Crespo, Olaf Kruse, Poul-Erik Jensen, Michael Schroda, Alison G. Smith, Stéphane D. Lemaire

algal biotechnology
Chlamydomonas reinhardtii
modular cloning
synthetic biology

Photocatalytic Production of Bisabolene from Green Microalgae Mutant: Process Analysis and Kinetic Modeling

Article Year: 2018

Authors: Irina Harun, Ehecatl Antonio Del Rio-Chanona, Jonathan L. Wagner, Kyle J. Lauersen, Dongda Zhan, Klaus Hellgardt

Hydrocarbons
Carbon
Biomass
Light Biofuels

Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum

Article Year: 2018

Authors: Nadja A. Henke, Julian Wichmann, Thomas Baier, Jonas Frohwitter, Kyle J. Lauersen, Joe M. Risse, Petra Peters-Wendisch, Olaf Kruse, Volker F. Wendisch

patchoulol
Corynebacterium glutamicum
sesquiterpene
metabolic engineering
Algae
Escherichia coli

Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii

Journal paper Year: 2018

Authors: Thomas Baier, Julian Wichmann, Olaf Kruse, Kyle J. Lauersen

Chlamydomonas reinhardtii
Microalgae

Tailored carbon partitioning for phototrophic production of (E)-α-bisabolene from the green microalga Chlamydomonas reinhardtii

Article Year: 2018

Authors: Julian Wichmann, Thomas Baier, Eduard Wentnagel, Kyle J. Lauersen, Olaf Kruse

Microalgae
Chlamydomonas reinhardtii
Terpenoids
Bisabolene
amiRNA

Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii

Article Year: 2016

Authors: Kyle J. Lauersen, Thomas Baier, Julian Wichmann, Robin Wördenweber, Jan H. Mussgnug, Wolfgang Hübner, Thomas Huser, Olaf Kruse

Microalgae
Chlamydomonas reinhardtii
Terpenoids
Sesquiterpenoids
patchoulol

Peroxisomal microbodies are at the crossroads of acetate assimilation in the green microalga Chlamydomonas reinhardtii

Article Year: 2016

Authors: Kyle J. Lauersen, Rémi Willamme, Nadine Coosemans, Marine Joris, Olaf Kruse, Claire Remacle

Glyoxylate cycle
Localization
microbodies
Isocitrate lyase
Acetate assimilation
Chlamydomonas reinhardtii

Investigating the dynamics of recombinant protein secretion from a microalgal host

Article Year: 2015

Authors: Kyle J. Lauersen, Isabel Hubera, Julian Wichmann, Thomas Baiera, Andreas Leite, Volker Gaukel, Viktor Kartushin, Anke Rattenholl, Christian Steinweg, Lenavon Riesen, Clemens Posten, Dirk Lütkemeyer, Jan H. Mussgnug, Olaf Kruse

Microalgae
Lolium perenne ice-binding protein
recombinant protein secretion
Flat-panel photobioreactor
Wave-bag culture
Chlamydomonas reinhardtii

Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit

Article Year: 2015

Authors: Kyle J. Lauersen, Olaf Kruse, Jan H. Mussgnug

Microalga
Chlamydomonas reinhardtii
Recombinant proteins
Fluorescent proteins
Luciferase
Protein secretion

Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production

Open access article Year: 2013

Authors: Christian Bogen, Arwa Al-Dilaimi, Andreas Albersmeier, Julian Wichmann, Michael Grundmann, Oliver Rupp, Kyle J. Lauersen, Olga Blifernez-Klassen, Jörn Kalinowski, Alexander Goesmann, Jan H. Mussgnug, Olaf Kruse

M. neglectum genome
Biofuels
Lipid metabolism
Neutral lipid accumulation

Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii

Article Year: 2013

Authors: Kyle J. Lauersen, Tara L. Vanderveer, Hanna Berger, Isabell Kaluza, Jan H. Mussgnug, Virginia K. Walker, Olaf Kruse

Microalga
Lolium perenne ice-binding protein
High-value recombinant protein production
Chlamydomonas reinhardtii
Secretion

Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii

Article Year: 2013

Authors: Kyle J. Lauersen, Hanna Berger, Jan H. Mussgnug, Olaf Kruse

Microalgae
Enhanced nuclear gene expression
Chlamydomonas reinhardtii
High-value recombinant protein production

Expression and characterization of an antifreeze protein from the perennial rye grass, Lolium perenne

Article Year: 2011

Authors: Kyle J. Lauersen, Alan Brown, Adam Middleton, Peter L. Davies, Virginia K.Walker

Antifreeze protein
Lolium perenne
Perennial ryegrass
Thermal hysteresis
Ice recrystallization inhibition
Ice-binding proteins
Freezing stress response
Plant antifreeze protein
Freezing tolerance

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