Kyle J. Lauersen

Principal Investigator

Assistant Professor

Location:

Ibn Al Haytham Building 2 - Office 4275

Biography

Dr. Kyle J. Lauersen is an Assistant Professor and principal investigator of the SSB group at KAUST. Kyle is from Kingston, Ontario Canada where he did his undergraduate, masters and teaching degrees at Queen’s University. Kyle was awarded a full scholarship by the CLIB Graduate Cluster to conduct his Doctorate (Dr. rer. Nat.) at the Center for Biotechnology as part of the Faculty of Biology at Bielefeld University, Germany. Kyle’s previous role was as a Postdoctoral research fellow in the Algae Biotechnology and Bioenergy research group of Prof. Dr. Olaf Kruse at Bielefeld University as part of the EU Horizon 2020 funded project Photofuel (http://www.photofuel.eu/home.php). Kyle joined the faculty of KAUST as a professor in August 2019 in order to work towards the translation of synthetic biology and biotechnology with a focus on microalgal technologies into sustainable biotechnological processes. 

Research Interests

Focus on green biotechnology with the main research theme of engineering algae to be green cell factories. Kyle is interested in promoting biotechnology applications that endeavor to be sustainable in scope, whether converting waste carbon streams like CO2 into products through algae, solar energy conversion into biomass, or the use of alternative carbon substrates as feedstocks for bio-processes. 

Selected Publications

Perozeni, F., Cazzaniga, S., Baier, T., Zanoni, F., Zoccatelli, G., Lauersen, K.J., Wobbe, L., Ballottari, M., 2020. Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii. Plant Biotechnol. J. pbi.13364. https://doi.org/10.1111/pbi.13364

Wichmann, J., Lauersen, K.J., Kruse, O., 2020. Green algal hydrocarbon metabolism is an exceptional source of sustainable chemicals. Curr. Opin. Biotechnol. 61, 28–37. https://doi.org/10.1016/j.copbio.2019.09.019

Jaeger, D., Baier, T., Lauersen, K.J., 2019. Intronserter, an advanced online tool for design of intron containing transgenes. Algal Res. 42, 101588. https://doi.org/10.1016/j.algal.2019.101588

Durante, L., Hübner, W., Lauersen, K.J., Remacle, C., 2019. Characterization of the GPR1/FUN34/YaaH protein family in the green microalga Chlamydomonas suggests their role as intracellular membrane acetate channels. Plant Direct 3, e00148. https://doi.org/10.1002/pld3.148

Lauersen, K.J., 2019. Eukaryotic microalgae as hosts for light-driven heterologous isoprenoid production. Planta 249, 155–180. https://doi.org/10.1007/s00425-018-3048-x

Perozeni, F., Cazzaniga, S., Baier, T., Zanoni, F., Zoccatelli, G., Lauersen, K.J., Wobbe, L., Ballottari, M., 2019. Turning a green alga red: engineering astaxanthin biosynthesis by intragenic pseudogene revival in Chlamydomonas reinhardtii. bioRxiv 535989. https://doi.org/10.1101/535989

Baier, T., Kros, D., Feiner, R.C., Lauersen, K.J., Müller, K.M., Kruse, O., 2018a. Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2547–2557. https://doi.org/10.1021/acssynbio.8b00226

Yunus, I.S., Wichmann, J., Wördenweber, R., Lauersen, K.J., Kruse, O., Jones, P.R., 2018. Synthetic metabolic pathways for photobiological conversion of CO2 into hydrocarbon fuel. Metab. Eng. 49, 201–211. https://doi.org/10.1016/j.ymben.2018.08.008

Lauersen, K.J., Wichmann, J., Baier, T., Kampranis, S.C., Pateraki, I., Møller, B.L., Kruse, O., 2018. Phototrophic production of heterologous diterpenoids and a hydroxy-functionalized derivative from Chlamydomonas reinhardtii. Metab. Eng. 49, 116–127. https://doi.org/10.1016/j.ymben.2018.07.005

Crozet, P., Navarro, F.J., Willmund, F., Mehrshahi, P., Bakowski, K., Lauersen, K.J., Pérez-Pérez, M.-E., Auroy, P., Gorchs Rovira, A., Sauret-Gueto, S., Niemeyer, J., Spaniol, B., Theis, J., Trösch, R., Westrich, L.-D., Vavitsas, K., Baier, T., Hübner, W., de Carpentier, F., Cassarini, M., Danon, A., Henri, J., Marchand, C.H., de Mia, M., Sarkissian, K., Baulcombe, D.C., Peltier, G., Crespo, J.-L., Kruse, O., Jensen, P.-E., Schroda, M., Smith, A.G., Lemaire, S.D., 2018. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086. https://doi.org/10.1021/acssynbio.8b00251

Harun, I., Del Rio-Chanona, E.A., Wagner, J.L., Lauersen, K.J., Zhang, D., Hellgardt, K., 2018. Photocatalytic Production of Bisabolene from Green Microalgae Mutant: Process Analysis and Kinetic Modeling. Ind. Eng. Chem. Res. 57, 10336–10344. https://doi.org/10.1021/acs.iecr.8b02509

Henke, N., Wichmann, J., Baier, T., Frohwitter, J., Lauersen, K., Risse, J., Peters-Wendisch, P., Kruse, O., Wendisch, V., 2018. Patchoulol Production with Metabolically Engineered Corynebacterium glutamicum. Genes (Basel). 9, 219. https://doi.org/10.3390/genes9040219

Baier, T., Wichmann, J., Kruse, O., Lauersen, K.J., 2018b. Intron-containing algal transgenes mediate efficient recombinant gene expression in the green microalga Chlamydomonas reinhardtii. Nucleic Acids Res. 46, 6909–6919. https://doi.org/10.1093/nar/gky532

Wichmann, J., Baier, T., Wentnagel, E., Lauersen, K.J., Kruse, O., 2018. Tailored carbon partitioning for phototrophic production of (E)-α-bisabolene from the green microalga Chlamydomonas reinhardtii. Metab. Eng. 45, 211–222. https://doi.org/10.1016/j.ymben.2017.12.010

Lauersen, K.J., Baier, T., Wichmann, J., Wördenweber, R., Mussgnug, J.H., Hübner, W., Huser, T., Kruse, O., 2016a. Efficient phototrophic production of a high-value sesquiterpenoid from the eukaryotic microalga Chlamydomonas reinhardtii. Metab. Eng. 38, 331–343. https://doi.org/10.1016/j.ymben.2016.07.013

Lauersen, K.J., Willamme, R., Coosemans, N., Joris, M., Kruse, O., Remacle, C., 2016b. Peroxisomal microbodies are at the crossroads of acetate assimilation in the green microalga Chlamydomonas reinhardtii. Algal Res. 16, 266–274. https://doi.org/10.1016/j.algal.2016.03.026

Lauersen, K.J., Huber, I., Wichmann, J., Baier, T., Leiter, A., Gaukel, V., Kartushin, V., Rattenholl, A., Steinweg, C., von Riesen, L., Posten, C., Gudermann, F., Lütkemeyer, D., Mussgnug, J.H.J.H., Kruse, O., 2015a. Investigating the dynamics of recombinant protein secretion from a microalgal host. J. Biotechnol. 215, 62–71. https://doi.org/10.1016/j.jbiotec.2015.05.001

Lauersen, K.J., Kruse, O., Mussgnug, J.H., 2015b. Targeted expression of nuclear transgenes in Chlamydomonas reinhardtii with a versatile, modular vector toolkit. Appl. Microbiol. Biotechnol. 99, 3491–3503. https://doi.org/10.1007/s00253-014-6354-7

Bogen, C., Al-Dilaimi, A., Albersmeier, A., Wichmann, J., Grundmann, M., Rupp, O., Lauersen, K.J., Blifernez-Klassen, O., Kalinowski, J., Goesmann, A., Mussgnug, J.H., Kruse, O., 2013. Reconstruction of the lipid metabolism for the microalga Monoraphidium neglectum from its genome sequence reveals characteristics suitable for biofuel production. BMC Genomics 14, 926. https://doi.org/10.1186/1471-2164-14-926

Lauersen, K.J., Vanderveer, T.L., Berger, H., Kaluza, I., Mussgnug, J.H., Walker, V.K., Kruse, O., 2013b. Ice recrystallization inhibition mediated by a nuclear-expressed and -secreted recombinant ice-binding protein in the microalga Chlamydomonas reinhardtii. Appl. Microbiol. Biotechnol. 97, 9763–9772. https://doi.org/10.1007/s00253-013-5226-x

Lauersen, K.J., Berger, H., Mussgnug, J.H., Kruse, O., 2013a. Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii. J. Biotechnol. 167, 101–110. https://doi.org/http://dx.doi.org/10.1016/j.jbiotec.2012.10.010

Lauersen, K.J., Brown, A., Middleton, A., Davies, P.L., Walker, V.K., 2011. Expression and characterization of an antifreeze protein from the perennial rye grass, Lolium perenne. Cryobiology 62, 194–201. https://doi.org/10.1016/j.cryobiol.2011.03.003

Research Interests Keywords

Synthetic Biology Algal Biotechnology Sustainability Waste-stream Reutilization